同仁化学Hampton蛋白结晶试剂盒TMAO/HR2-857| 日本DOJINDO

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Products > Optimize Reagents > Optimize – Cryoprotectants > TMAO

TMAO

Hampton蛋白结晶试剂盒TMAO/HR2-857

CAT NO

HR2-857

NAME

4.0 M TMAO

DESCRIPTION

1 mL

PRICE

$26.00

支持材料

Hampton蛋白结晶试剂盒TMAO/HR2-857 HR2-857 4.0 M TMAO SDS

应用

  • Water soluble cryoprotectant
  • 水溶性冷冻保护剂

特征

  • A very versatile cryoprotectant for macromolecular crystals
  • Protein stabilizer
  • TMAO can be a viable alternative when conventional cryoprotectants fail
  • Low viscosity and low surface tension
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 一种非常通用的大分子晶体冷冻保护剂
    蛋白质稳定剂
    当传统冷冻保护剂失效时,TMAO 可能是一种可行的替代方案
    低粘度和低表面张力
    无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Trimethylamine N-oxide dihydrate (TMAO)

Formulated from TMAO dihydrate in Type 1 lab water (deionized) and sterile filtered (0.22 micron)

Synonyms: TMANO, TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
Mr 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
Purity ≥ 98.0%
Measured pH range: 9.2 – 10.6 at 25°C
Measured Conductivity Range: 5.6 – 96.6 µS/cm at 25°C
Measured Refractive Index Range: 1.37660 – 1.37804 at 20°C

When using TMAO as a cryoprotectant, try soaking the crystals in 4.0 M TMAO for 10 seconds and then cryo cool directly in the cryo stream.

由 1 型实验室用水(去离子)和无菌过滤(0.22 微米)中的 TMAO 二水合物配制而成

同义词:TMANO、TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
纯度≥98.0%
测得的 pH 值范围:25°C 时 9.2 – 10.6
测得的电导率范围:25°C 时 5.6 – 96.6 µS/cm
测得的折射率范围:20°C 时为 1.37660 – 1.37804

当使用 TMAO 作为冷冻保护剂时,尝试将晶体浸泡在 4.0 M TMAO 中 10 秒,然后直接在冷冻流中冷冻冷却。

相关项目

  • CryoPro

参考

1. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Christoph Mueller-Dieckmann, Brice Kauffmann and Manfred S. Weiss. J. Appl. Cryst. (2011). 44, 433–436 doi:10.1107/S0021889811000045.

1. 三甲胺 N-氧化物作为大分子晶体学中的多功能冷冻保护剂。 Christoph Mueller-Dieckmann、Brice Kauffmann 和 Manfred S. Weiss。 J.应用。 水晶。 (2011)。 44, 433–436 doi:10.1107/S0021889811000045。

同仁化学Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词: Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围:7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围:25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围:25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率:20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围:25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率:20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents
  • 盐酸
    StockOptions Tris 缓冲套件
    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田,Y; 比曼,台湾; 罗德里克,SL; 蛋白质 28, 298-300, 1997。

同仁化学Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 10 micron/HR4-994/HR4-995/HR4-999/HR4-997| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 10 micron

Mounted CryoLoop™ – 10 micron

Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 10 micron/HR4-994/HR4-995/HR4-999/HR4-997
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 10 micron/HR4-994/HR4-995/HR4-999/HR4-997
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 10 micron/HR4-994/HR4-995/HR4-999/HR4-997

CAT NO

HR4-994

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.025-0.05 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-995

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-997

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-999

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$91.00

应用

  • Cryocrystallography
  • 低温晶体学

特征

  • Synthetic CryoLoop on stainless steel MicroTube
  • MicroTube specially engineered with EasySnap notches
  • 不锈钢 MicroTube 上的合成 CryoLoop
    MicroTube 特别设计,带有 EasySnap 凹槽

描述

Mounted CryoLoops with 10 micron diameter nylon. The 10 micron Mounted CryoLoop shows less background diffraction than the 20 micron Mounted CryoLoop. Please note that the 10 micron Mounted CryoLoops are very flexible due to their small diameter. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

安装有 10 微米直径尼龙的 CryoLoops。 10 微米安装的 CryoLoop 显示的背景衍射比 20 微米安装的 CryoLoop 少。请注意,10 微米安装的 CryoLoop 因其小直径而非常灵活。这些尼龙环与中空不锈钢 MicroTubes™ 粘合,用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 MicroTube 的长度为 24 毫米,专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube,只需将 MicroTube 卡入所需长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。这些尼龙环显示出最小的衍射,很薄,可以快速冷冻,坚固且符合空气动力学。

大多数数据收集几何结构更喜欢或要求帽底和梁之间有 22 毫米的长度。对于使用 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 的 22 mm 总长度,将 MicroTube 从底部的第二个凹口处卡入,形成 18 mm 的 MicroTube 长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。对于 22 mm 以上的长度,使用 CrystalCap Copper、CrystalCap Copper HT、CrystalCap ALS Copper HT 将 MicroTube 卡在从底部算起的第五个槽口(最靠近 CryoLoop 的槽口),并使用环氧树脂或类似粘合剂固定到 CrystalCap。

参考HR4-994 Mounted CryoLoop 0.025 – 0.05 mm

1. A novel approach to micro-sample X-ray powder diffraction using nylon loops. Reibenspies and Bhuvanesh. J. Appl. Cryst. (2003). 36, 1480-1481.

1. 一种使用尼龙环进行微量样品 X 射线粉末衍射的新方法。 Reibenspies 和 Bhuvanesh。 J.应用。 水晶。 (2003 年)。 36, 1480-1481。

同仁化学Hampton蛋白结晶试剂盒Epoxy/HR4-347| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Cryocrystallography > CryoLoops > Epoxy

Epoxy

Hampton蛋白结晶试剂盒Epoxy/HR4-347

CAT NO

HR4-347

NAME

Double/Bubble Epoxy

DESCRIPTION

5 pack

PRICE

$15.00

支持材料

Hampton蛋白结晶试剂盒Epoxy/HR4-347 HR4-347 Double/Bubble Epoxy SDS

应用

  • Sealing capillary tubes
  • Securing MicroTubes into CrystalCaps
  • Securing CryoLoops into MicroTubes
  • 密封毛细管
    将微管固定在 CrystalCaps 中
    将 CryoLoop 固定到 MicroTube 中

特征

  • Fast setting
  • Excellent cohesive strength
  • 快速设置
    优异的内聚强度

描述

Double/Bubble® Epoxy HR4-347 extra fast setting with a work time of 3 to 5 minutes. The preferred epoxy for cryocrystallography. Sold as a pack of 5. Hardman Double/Bubble Fast-Setting Epoxy is an extra fast setting, two part, room temperature curing epoxy adhesive. It has a convenient mix ratio, excellent cohesive strength and bonds to most substrates including metal, wood, concrete, ceramics and many plastics. The Double/Bubble package is a handy, dual-pouch, one-shot, job-size packaging concept. Fills gaps, will not shrink. Contains no solvents. Two part epoxy in a single, dual-pouch 3.5 gram packet.

To use:
1. Fold Double/Bubble packet
2. Cut or tear to open end
3. Squeeze out adhesive
4. Mix parts A and B together with supplied spatula

Supplied as 5 packets with 5 spatulas.

Double/Bubble® Epoxy HR4-347 超快速设置,工作时间为 3 至 5 分钟。 低温晶体学的首选环氧树脂。 一包 5 件出售。 Hardman Double/Bubble Fast-Setting Epoxy 是一种超快速固化的两部分室温固化环氧树脂粘合剂。 它具有方便的混合比、出色的内聚强度以及与大多数基材的粘合性,包括金属、木材、混凝土、陶瓷和许多塑料。 Double/Bubble 包装是一种方便、双袋、一次性、工作大小的包装概念。 填补空白,不会缩小。 不含溶剂。 单双袋 3.5 克小包中的两部分环氧树脂。

要使用:
1.折叠双/气泡包
2. 切开或撕开端
3.挤出粘合剂
4. 用随附的抹刀将 A 部分和 B 部分混合在一起

以 5 包 5 把抹刀的形式提供。

Color/Appearance: Amber
Product Name: Hardman DOUBLE/BUBBLE Extra-Fast Set Epoxy Red Package 3.5 g Packet 1 part or 2: 2 part
Base Chemistry: Part A: Epoxy; Part B: Polymer
Handling strength: 15 to 30 minutes at 25°C
Cure Time: 24h at 25°C
Durometer/Hardness: 80 D at 25°C
Flash Point: Part A: 190.5°C; Part B: 132.2°C
Mix Ratio: 1:1
Peel Strength: 1.8 pli at 25°C
Service Temperature: Up to 82°C
Shear Strength Psi: 3,000 at 25°C
Specific Gravity: Mixed: 1.16 at 25°C
Viscosity Cp: Mixed: 40,000 at 25°C
Work Life/Pot Life: 3 minutes

相关项目HR4-347 Double Bubble Epoxy

  • 18 mm Mounted CryoLoop™ – 20 micron

同仁化学Hampton蛋白结晶试剂盒L-Proline/HR2-775| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > L-Proline

L-Proline

Hampton蛋白结晶试剂盒L-Proline/HR2-775

CAT NO

HR2-775

NAME

1.0 M L-Proline

DESCRIPTION

100 mL

PRICE

$81.00

支持材料

Hampton蛋白结晶试剂盒L-Proline/HR2-775 HR2-775 L-Proline SDS

应用

  • Crystallization grade L-Proline for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 L-脯氨酸

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述L-Proline

Synonyms: (S)-Pyrrolidine-2-carboxylic acid
C5H9NO2
Mr 115.13
CAS Number [147-85-3]
EC Number 205-702-2
Beilstein Registry Number 80810
Merck 14,7780
RTECS TW3584000
MDL Number MFCD00064318
PubChem Substance ID 24898097
Purity ≥ 99.0%

Measured pH range: 6.1 – 6.5 at 25°C
Measured Conductivity Range: 4.7 – 32.9 µS/cm at 25°C
Measured Refractive Index Range: 1.35255 – 1.35280 at 20°C

同义词:(S)-Pyrrolidine-2-carboxy acid
C5H9NO2
115.13 先生
CAS 编号 [147-85-3]
欧盟编号 205-702-2
贝尔斯坦注册号 80810
默克 14,7780
RTECS TW3584000
MDL 编号 MFCD00064318
PubChem 物质 ID 24898097
纯度≥99.0%

测得的 pH 值范围:25°C 时为 6.1 – 6.5
测得的电导率范围:25°C 时为 4.7 – 32.9 µS/cm
测得的折射率范围:20°C 时为 1.35255 – 1.35280

L-Proline

同仁化学Hampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Sodium malonate

Sodium malonate

Hampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807

CAT NO

HR2-747

NAME

3.4 M Sodium malonate pH 4.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-749

NAME

3.4 M Sodium malonate pH 5.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-751

NAME

3.4 M Sodium malonate pH 6.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-707

NAME

3.4 M Sodium malonate pH 7.0

DESCRIPTION

200 mL

PRICE

$107.00

CAT NO

HR2-807

NAME

3.4 M Sodium malonate pH 8.0

DESCRIPTION

200 mL

PRICE

$107.00

支持材料

Hampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-747 Sodium malonate pH 4.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-749 Sodium malonate pH 5.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-751 Sodium malonate pH 6.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-707 Sodium malonate pH 7.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 HR2-807 Sodium malonate pH 8.0 SDSHampton蛋白结晶试剂盒Sodium malonate/HR2-747/HR2-749/HR2-751/HR2-707/HR2-807 pH/Dilution Table for Sodium Malonate

应用

  • Crystallization grade Sodium malonate for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级丙二酸钠

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

The Sodium malonate offered by Hampton Research is Malonic acid (CAS Number 141-82-2) titrated to pH 4.0-8.0 using Sodium hydroxide as described in the publication “A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.”. In this same publication, Sodium malonate was reported to be more successful than any other salt, resulting in the crystallization of 19 of the 23 macromolecules, almost twice as effective as the next most successful salt, which was a draw between sodium acetate, sodium tartrate, sodium formate, and ammonium sulfate. The high success rate of Sodium malonate in producing crystals was even more impressive when an overall unique success rate with individual macromolecules was considered.

The use of Sodium malonate in place of Ammonium sulfate has been reported in one instance to improve the reproducibility of crystals and improve resistance to physicochemical shocks. Sodium malonate also acted as a good cryoprotectant (Xing and Xu 2003).

One may combine the four different sodium malonate stocks to create a pH versus sodium malonate concentration grid for screening or optimization. Refer to the pH/Dilution Table for Sodium malonate.

Based on the structure of malonic acid titrated with sodium hydroxide, there are no chiral carbons so it is not characterized as having enantiomeric structures, such as D or L.

Hampton Research 提供的丙二酸钠是使用氢氧化钠滴定至 pH 4.0-8.0 的丙二酸(CAS 编号 141-82-2),如出版物“用于大分子结晶的盐的比较。Alexander McPherson。蛋白质科学( 2001), 10:418-422”。在同一份出版物中,据报道丙二酸钠比任何其他盐更成功,导致 23 种大分子中的 19 种结晶,几乎是下一个最成功的盐的两倍,后者是乙酸钠和酒石酸钠之间的抽吸、甲酸钠和硫酸铵。当考虑到单个大分子的整体独特成功率时,丙二酸钠在生产晶体中的高成功率更加令人印象深刻。

据报道,在一个实例中使用丙二酸钠代替硫酸铵可提高晶体的再现性并提高对物理化学冲击的抵抗力。丙二酸钠也是一种很好的冷冻保护剂(Xing and Xu 2003)。

可以将四种不同的丙二酸钠库存组合起来,以创建一个 pH 值与丙二酸钠浓度网格,以进行筛选或优化。请参阅丙二酸钠的 pH/稀释表。

根据用氢氧化钠滴定的丙二酸的结构,没有手性碳,因此不具有对映异构体结构的特征,例如 D 或 L。

Synonyms: Propanedioic acid or Malonic acid
C3H4O4 (before titration with NaOH)
Mr 104.06 (before titration with NaOH)
CAS Number 141-82-2
EC Number 205-503-0
Beilstein Registry Number 1751370
Merck 14,5710
RTECS OO0175000
MDL Number MFCD00002707
Purity > 99.0%
pKa1 2.8
pKa2 5.7

HR2-747 Measured Conductivity Range: 73.3 – 75.3 mS/cm at 25°C
HR2-747 Measured Refractive Index Range: 1.38365 – 1.38425 at 20°C

HR2-749 Measured Conductivity Range: 77.2 – 78.0 mS/cm at 25°C
HR2-749 Measured Refractive Index Range: 1.39264 – 1.39300 at 20°C

HR2-751 Measured Conductivity Range: 73.4 – 75.2 mS/cm at 25°C
HR2-751 Measured Refractive Index Range: 1.39938 – 1.39973 at 20°C

HR2-707 Measured Conductivity Range: 71.0 – 73.7 mS/cm at 25°C
HR2-707 Measured Refractive Index Range: 1.40090 – 1.40098 at 20°C

HR2-807 Measured Conductivity Range: 71.8 – 75.0 mS/cm at 25°C
HR2-807 Measured Refractive Index Range: 1.40106 – 1.40115 at 20°C

参考Sodium malonate

1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

2. Crystallization of the PX domain of cytokine-independent survival kinase (CISK): improvement of crystal quality for X-ray diffraction with sodium malonate. Yi Xing and Wenqing Xu. Acta Cryst. (2003). D59, 1816-1818.

3. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko. Acta Cryst. (2003). D59, 2356-2358.

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase. Kim et al Acta Cryst. (2007). F63, 579–581.

1. 大分子结晶盐的比较。 亚历山大·麦克弗森。 蛋白质科学(2001),10:418-422。

2. 不依赖细胞因子的存活激酶 (CISK) 的 PX 结构域的结晶:用丙二酸钠改善 X 射线衍射的晶体质量。 艺兴和徐文清。 晶体学报。 (2003 年)。 D59,1816-1818。

3. 丙二酸盐:一种用于盐生大分子晶体的多功能冷冻保护剂和稳定剂。 T. Holyoak、T. D. Fenn、M. A. Wilson、A. G. Moulin、D. Ringe 和 G. A. Petsko。 晶体学报。 (2003 年)。 D59,2356-2358。

4. Est25 的纯化、结晶和初步晶体学分析:一种酮洛芬特异性激素敏感性脂肪酶。 Kim等人Acta Cryst。 (2007 年)。 F63, 579–581。

同仁化学Hampton蛋白结晶试剂盒Tacsimate pH4&5&6&7&8&9/HR2-823/HR2-755/HR2-829/HR2-813| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Hampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813

CAT NO

HR2-823

NAME

100% Tacsimate pH 4.0

DESCRIPTION

200 mL

PRICE

$120.00

CAT NO

HR2-825

NAME

100% Tacsimate pH 5.0

DESCRIPTION

200 mL

PRICE

$120.00

CAT NO

HR2-827

NAME

100% Tacsimate pH 6.0

DESCRIPTION

200 mL

PRICE

$120.00

CAT NO

HR2-755

NAME

100% Tacsimate pH 7.0

DESCRIPTION

200 mL

PRICE

$120.00

CAT NO

HR2-829

NAME

100% Tacsimate pH 8.0

DESCRIPTION

200 mL

PRICE

$120.00

CAT NO

HR2-813

NAME

100% Tacsimate pH 9.0

DESCRIPTION

200 mL

PRICE

$120.00

支持材料

Hampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 What is Tacsimate?Hampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 Mixing Tacsimate Stocks to Screen PhHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-823 Tacsimate 100% solution pH 4.0 SDSHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-825 Tacsimate 100% solution pH 5.0 SDSHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-827 Tacsimate 100% solution pH 6.0 SDSHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-755 Tacsimate 100% solution pH 7.0 SDSHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-829 Tacsimate 100% solution pH 8.0 SDSHampton蛋白结晶试剂盒Tacsimate pH4&#038;5&#038;6&#038;7&#038;8&#038;9/HR2-823/HR2-755/HR2-829/HR2-813 HR2-813 Tacsimate 100% solution pH 9.0 SDS

应用

  • Crystallization grade Tacsimate for formulating screens or for optimization
  • 结晶级 Tacsimate 用于配制屏幕或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

To screen pH as a crystallization variable using Tacsimate one can mix together different ratios of different pH Tacsimate solutions. Refer to the pdf “Mixing Tacsimate Stocks to Screen pH” on this page. Or, one can add a buffer to the crystallizaiton reagent containing Tacsimate. Recommended final buffer concentration in the reagent well is 0.05 to 0.1 M.

Tacsimate
A pH titrated mixture of organic acids. 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic. pH adjusted using NaOH

Measured pH of 100% Tacsimate is 4.0 at 25°C
Measured pH of 100% Tacsimate is 5.0 at 25°C
Measured pH of 100% Tacsimate is 6.0 at 25°C
Measured pH of 100% Tacsimate is 7.0 at 25°C
Measured pH of 100% Tacsimate is 8.0 at 25°C
Measured pH of 100% Tacsimate is 9.0 at 25°C

为了使用 Tacsimate 将 pH 值筛选为结晶变量,可以将不同比例的不同 pH 值的 Tacsimate 溶液混合在一起。 请参阅本页上的 pdf“混合 Tacsimate 库存以筛选 pH 值”。 或者,可以将缓冲液添加到含有 Tacsimate 的结晶试剂中。 试剂孔中推荐的最终缓冲液浓度为 0.05 至 0.1 M。

战术
pH 值滴定的有机酸混合物。 1.8305 M 丙二酸、0.25 M 柠檬酸三铵、0.12 M 琥珀酸、0.3 M DL-苹果酸、0.4 M 三水乙酸钠、0.5 M 甲酸钠、0.16 M 酒石酸氢铵。 使用 NaOH 调节 pH

在 25°C 时测得的 100% Tacsimate 的 pH 值为 4.0
在 25°C 时测得的 100% Tacsimate 的 pH 值为 5.0
在 25°C 时测得的 100% Tacsimate 的 pH 值为 6.0
在 25°C 时测得的 100% Tacsimate 的 pH 值为 7.0
在 25°C 时测得的 100% Tacsimate 的 pH 值为 8.0
在 25°C 时测得的 100% Tacsimate 的 pH 值为 9.0

Mixture of organic acids. Malonic acid, Ammonium citrate tribasic, Succinic acid, DL-Malic acid, Sodium acetate trihydrate, Sodium formate, and Ammonium tartrate dibasic

有机酸的混合物。 丙二酸、柠檬酸三铵、琥珀酸、DL-苹果酸、醋酸钠三水合物、甲酸钠和酒石酸氢铵

参考

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387–406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney and Alexander McPherson. Acta Cryst. (2007). D63, 310–318.

3. Structure of the catalytic and ubiquitin-associated domains of the protein kinase MARK/Par-1. Eckhard Mandelkow et al. Structure 14, 173-183, February 2006.

4. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

5. Crystallization of a newly discovered histidine acid phosphatase from Francisella tularensis. Richard L. Felts, Thomas J. Reilly, Michael J. Calcutt, and John J. Tanner. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 January 1; 62(Pt 1): 32–35.

6. Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila. Petermann et al, FEBS Letters Volume 584, Issue 4, Pages 733-738 (19 February 2010).

1. 寻找灵丹妙药:结晶大分子的另一种策略。亚历山大·麦克弗森和鲍勃·库德尼。结构生物学杂志 156 (2006) 387–406。

2. 蛋白质结晶的新策略:X 射线衍射验证。 Steven B. Larson、John S. Day、Robert Cudney 和 Alexander McPherson。晶体学报。 (2007 年)。 D63,310-318。

3. 蛋白激酶 MARK/Par-1 的催化和泛素相关结构域的结构。 Eckhard Mandelkow 等人。结构 14, 173-183,2006 年 2 月。

4. 大分子结晶盐的比较。亚历山大·麦克弗森。蛋白质科学(2001),10:418-422。

5. 从土拉弗朗西斯菌中新发现的组氨酸酸性磷酸酶的结晶。 Richard L. Felts、Thomas J. Reilly、Michael J. Calcutt 和 John J. Tanner。 Acta Crystallogr Sect F Struct Biol Cryst Commun。 2006 年 1 月 1 日; 62(第 1 篇):32-35。

6. 嗜肺军团菌的亚铁转运体 FeoB 的 GTPase 和 GDI 结构域。 Petermann 等人,FEBS Letters 第 584 卷,第 4 期,第 733-738 页(2010 年 2 月 19 日)。

同仁化学Hampton蛋白结晶试剂盒Tryptone/HR2-835| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Tryptone

Tryptone

Hampton蛋白结晶试剂盒Tryptone/HR2-835

CAT NO

HR2-835

NAME

10% w/v Tryptone

DESCRIPTION

100 mL

PRICE

$94.00

支持材料

Hampton蛋白结晶试剂盒Tryptone/HR2-835 HR2-835 10% w/v Tryptone SDS

应用

  • Crystallization grade Tryptone for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级胰蛋白胨

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tryptone

Synonyms: Peptone from casein
Microbiologically tested
CAS Number [91079-40-2]
EC Number: 293-428-4

Measured pH Range: 6.9 – 7.1 at 25°C
Measured Conductivity Range: 7.5 – 11.1 mS/cm at 25°C
Measured Refractive Index Range: 1.35040 – 1.35133 at 20°C

Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is rich in tryptophan. There are no detectable levels of carbohydrates in Tryptone. The enzymatic digest is pasteurized and spray dried prior to formulation in Type 1 laboratory water and sterile filtered (0.22 micron filter).

同义词:酪蛋白蛋白胨
微生物测试
CAS 编号 [91079-40-2]
欧盟编号:293-428-4

测得的 pH 值范围:25°C 时为 6.9 – 7.1
测得的电导率范围:25°C 时 7.5 – 11.1 mS/cm
测得的折射率范围:20°C 时为 1.35040 – 1.35133

胰蛋白胨是由蛋白酶胰蛋白酶消化酪蛋白形成的肽组合。 色氨酸富含色氨酸。 胰蛋白胨中没有可检测到的碳水化合物水平。 酶消化物经过巴氏杀菌和喷雾干燥,然后在 1 型实验室用水中进行配制并无菌过滤(0.22 微米过滤器)。

参考Tryptone

1. Progress in the Development of an Alternative Approach to Macromolecular Crystallization. S. B. Larson,J. S. Day, C. Nguyen, R. Cudney, and A. McPherson. Crystal Growth & Design 2008 Volume 8, No. 8 3038-3052.

1. 大分子结晶替代方法的开发进展。 S. B.拉尔森,J. S. Day、C. Nguyen、R. Cudney 和 A. McPherson。 晶体生长与设计 2008 年第 8 卷,第 8 期 3038-3052。

同仁化学Hampton蛋白结晶试剂盒Glycerol/HR2-623| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Organics (Non-Volatile) > Glycerol

Glycerol

Hampton蛋白结晶试剂盒Glycerol/HR2-623

 

CAT NO

HR2-623

NAME

100% Glycerol

DESCRIPTION

100 mL

PRICE

$84.00

支持材料

Hampton蛋白结晶试剂盒Glycerol/HR2-623 HR2-623 Glycerol SDS

应用

  • Crystallization grade Glycerol for formulating screens, optimization and cryoprotection
  • 结晶级甘油,用于配制筛选、优化和冷冻保护

特征

  • Gradually increasing the Glycerol concentration to 5 to 35% in the original crystallization reagent is often times an effective cryoprotectant.
  • 将原始结晶试剂中的甘油浓度逐渐增加到 5% 到 35% 通常是一种有效的冷冻保护剂。

描述Glycerol

Synonyms: 1,2,3-Propanetriol or Glycerin
HOCH2CH(OH)CH2OH
C3H8O3
Mr 92.09
CAS Number [56-81-5]
EC Number 200-289-5
Beilstein Registry Number 635685
RTECS MA8050000
MDL Number MFCD00004722
PubChem Substance ID 24895252
Purity ≥ 99.0% (GC)
pKa 14.2

Measured pH range of 100% Glycerol: 5.0 – 7.7 at 25°C
Measured Conductivity Range: 0.0 – 0.2 µS/cm at 25°C
Measured Refractive Index Range: 1.47124 – 1.47450 at 20°C

同义词:1,2,3-丙三醇或甘油
HOCH2CH(OH)CH2OH
C3H8O3
92.09 先生
CAS 编号 [56-81-5]
欧盟编号 200-289-5
贝尔斯坦注册号 635685
RTECS MA8050000
MDL 编号 MFCD00004722
PubChem 物质 ID 24895252
纯度 ≥ 99.0% (GC)
pKa 14.2

测得的 100% 甘油的 pH 值范围:25°C 时为 5.0 – 7.7
测量电导率范围:25°C 时 0.0 – 0.2 µS/cm
测得的折射率范围:20°C 时为 1.47124 – 1.47450

Al  ≤0.0005%
Ca ≤0.0005%
Cd ≤0.0001%
Cl  ≤0.001%
Cu ≤0.0005%
Fe ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
NH3 ≤0.05%
NH4+ ≤0.05%
Na ≤0.005%
P  ≤0.0005%
Pb ≤0.001%
SO4 ≤0.002%
Zn ≤0.0005%

参考Glycerol

1. Crystallization and Preliminary X-ray Analysis of the Auto-inhibited Twitchin Kinase. Hu, S-H; Lei, JY; Wilce, MCJ; Valenzuela, MRL; Benian, GM; Parker, MW; Kemp, BE. J Mol Biol 236, 1259- 1261, 1994.

1. 自抑制 Twitchin 激酶的结晶和初步 X 射线分析。 胡,S-H; 雷,JY; 威尔斯,MCJ; 瓦伦苏埃拉,MRL; 贝尼安,总经理; 帕克,兆瓦; 坎普,BE。 分子生物学杂志 236, 1259-1261, 1994。