SD/-Met/-Trp with Agar 酵母缺陷型培养基(琼脂)

SD/-Met/-Trp with Agar 酵母缺陷型培养基(琼脂)

货号: JP6388-5L 品牌: Jinpan 标签: 生化试剂

描述

SD/-Met/-Trp with Agar

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB; Minimal SD Base;Dropout (DO) Supplements氨基酸缺失混合物;

产品信息

产品名称 产品编号           规格              价格(元)    
SD/-Met/-Trp with Agar 酵母缺陷型培养基(琼脂)    JP6388-5L     10×0.5 L 1198

产品描述

酵母缺陷型培养基是由SD Base + DO Supplement,以添加琼脂和未添加琼脂两种方式混合而成的完全培养基,前者是固体培养基,后者是液体培养基(肉汤),用于选择培养含特异载体的酵母或进行杂交筛选。

Minimal SD Base,即Minimal Synthetical Defined Medium,是在无氨基酸酵母氮源基础(YNB)上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement,即氨基酸缺陷混合物(或省却成分添加剂)是缺乏一种或多种特定氨基酸的混合物。

SD/-Met/-Trp with Agar就是缺乏甲硫氨酸(Met)和色氨酸(Trp)两种成分的酵母缺陷型固体培养基,常用于双质粒酵母转化筛选和单/双杂交系统报告基因检测。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

培养基配制

1)   取一包粉末,全部加入500ml去离子水,搅拌混匀使其溶解。琼脂不能溶解直至高压灭菌后。

2)  重要:测试培养基pH是否为5.8,若有必要用弱酸或弱碱调整pH。

3)   121℃高压灭菌15min。冷却至~50℃后再倒板。

4)   室温下使得平板培养基凝固,用封口膜封好后倒置保存在4℃。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

附表1 酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
Rich Media

富集培养基

YPD Agar Medium JP6301-350G 70
YPD Medium JP6302-250G 50
YPDA Agar Medium JP6303-350G 70
YPDA Medium JP6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base JP6311-467G 46.7
Minimal SD Base JP6312-267G 26.7
Minimal SD Agar Base Gal/Raf JP6313-270G 54
Minimal SD Base Gal/Raf JP6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His JP6316-10G 1.29
DO Supplement -Leu JP6317-10G 1.29
DO Supplement -Trp JP6318-10G 1.29
DO Supplement -Ura JP6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu JP6320-10G 1.19
DO Supplement -His/-Ura JP6321-10G 1.27
DO Supplement -Leu/-Trp JP6322-10G 1.19
DO Supplement -Met/-Trp JP6323-10G 1.27
DO Supplement -Trp/-Ura JP6324-10G 1.27
DO Supplement -Trp/-His JP6325-10G 1.27
DO Supplement -Ura/-Leu JP6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp JP6331-10G 1.17
DO Supplement -His/-Leu/-Ura JP6332-10G 1.17
DO Supplement -His/-Trp/-Ura JP6333-10G 1.25
DO Supplement -Leu/-Met/-Trp JP6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura JP6335-10G 1.17
DO Supplement -Trp/-His/-Ade JP6336-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp    JP6341-10G        1.15
DO Supplement -His/-Leu/-Met/-Trp JP6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura JP6343-10G 1.15
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His Broth JP6361-5L 28
SD/-Leu Broth JP6363-5L 28
SD/-Trp Broth JP6365-5L 28
SD/-Ura Broth JP6367-5L 28
SD/-His/-Leu Broth JP6381-5L 28
SD/-His/-Ura Broth JP6383-5L 28
SD/-Leu/-Trp Broth JP6385-5L 28
SD/-Met/-Trp Broth JP6387-5L 28
SD/-Trp/-Ura Broth JP6389-5L 28
SD/-Trp/-His Broth JP6391-5L 28
SD/-Trp/-Ade Broth JP6393-5L 28
SD/-Ura/-Leu Broth JP6395-5L 28

上接附表1酵母培养基配制浓度表

培养基类型 名称 货号 终浓度(g/L)
SD + DO Broth酵母缺陷型完全培养基(肉汤) SD/-His/-Leu/-Trp Broth JP6411-5L         28
SD/-His/-Leu/-Ura Broth JP6413-5L 28
SD/-His/-Trp/-Ura Broth JP6415-5L 28
SD/-Leu/-Met/-Trp Broth JP6417-5L 28
SD/-Leu/-Trp/-Ura Broth JP6419-5L 28
SD/-Trp/-His/-Ade Broth JP6421-5L 28
SD/-Ade/-His/-Leu/-Trp Broth JP6431-5L 28
SD/-His/-Leu/-Met/-Trp Broth JP6433-5L 28
SD/-His/-Leu/-Trp/-Ura Broth JP6435-5L 28
SD + DO with Agar酵母缺陷型完全培养基(琼脂)  SD/-His with Agar JP6362-5L ——
SD/-Leu with Agar JP6364-5L ——
SD/-Trp with Agar JP6366-5L ——
SD/-Ura with Agar JP6368-5L ——
SD/-His/-Leu with Agar JP6382-5L ——
SD/-His/-Ura with Agar JP6384-5L ——
SD/-Leu/-Trp with Agar JP6386-5L ——
SD/-Met/-Trp with Agar JP6388-5L ——
SD/-Trp/-Ura with Agar JP6390-5L ——
SD/-Trp/-His with Agar JP6392-5L       ——
SD/-Trp/-Ade with Agar JP6394-5L ——
SD/-Ura/-Leu with Agar JP6396-5L ——
SD/-His/-Leu/-Trpwith Agar          JP6412-5L ——
SD/-His/-Leu/-Urawith Agar JP6414-5L ——
SD/-His/-Trp/-Urawith Agar JP6416-5L ——
SD/-Leu/-Met/-Trpwith Agar JP6418-5L ——
SD/-Leu/-Trp/-Urawith Agar JP6420-5L ——
SD/-Trp/-His/-Adewith Agar JP6422-5L ——
SD/-Ade/-His/-Leu/-Trpwith Agar JP6432-5L ——
SD/-His/-Leu/-Met/-Trpwith Agar JP6434-5L ——
SD/-His/-Leu/-Trp/-Urawith Agar JP6436-5L ——

 

  

 

 

规格信息

品牌:

Jinpan

CAS:

N/A

规格:

10×0.5 L

货期:

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