DO Supplement -Met/-Trp

DO Supplement -Met/-Trp

货号: JP6323-10G 品牌: Jinpan 标签: 生化试剂

描述

DO Supplement -Met/-Trp

产品标签

YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;

产品信息

  产品名称                                产品编号             规格            价格(元)   促销价(元) 
  DO Supplement -Met/-Trp  JP6323-10G  10g  418  318
  DO Supplement -Met/-Trp  JP6323-100G  100g  2518  1998

产品描述

酵母菌的质粒筛选通常基于营养缺陷型突变菌株的使用而开展,这些菌株在缺乏某种特定培养基组分(比如氨基酸,嘌呤或嘧啶)的情况下无法生长。当转化含有突变基因的质粒后使得阳性转化克隆能够在缺乏必需营养组分的培养基内生长,正因如此,省却成分培养基添加剂(DO medium supplements)能用来筛选转化的酵母培养物。

本品为缺乏蛋氨酸(Met)/色氨酸(Trp)的省却成分培养基添加剂,与Minimal SD base联合使用,配制成双组分缺陷的酵母菌选择性培养基,俗称双缺培养基(二缺培养基),用于双质粒酵母转化筛选。可用在外源基因在酵母中的表达、异源基因功能互补、酵母单杂交系统和酵母双杂交系统初始质粒转化免疫共沉淀、Y187和AH109酵母交配实验、接合型二倍体筛选。

保存与运输方法

保存:室温干燥保存,至少2年稳定。

运输:室温运输。

注意事项

为了您的安全和健康,请穿实验服并戴一次性手套操作。

相关产品

货号                 名称                                         规格               价格(元)  
JP6320-10G DO Supplement -His/-Leu 10g /
JP6321-10G DO Supplement -His/-Ura 10g /
JP6322-10G DO Supplement -Leu/-Trp 10g /
JP6323-10G DO Supplement -Met/-Trp 10g /
JP6324-10G DO Supplement -Trp/-Ura 10g /
JP6325-10G DO Supplement -Trp/-His 10g /
JP6326-10G DO Supplement -Ura/-Leu 10g /

表1 酵母培养基配制浓度表

培养基类型                                            名称                                                  货号                   终浓度(g/L)     
Rich Media

富集培养基

YPD Agar Medium JP6301-350G 70
YPD Medium JP6302-250G 50
YPDA Agar Medium JP6303-350G 70
YPDA Medium JP6304-250G 50
Minimal Media

基本培养基

Minimal SD Agar Base JP6311-467G 46.7
Minimal SD Base JP6312-267G 26.7
Minimal SD Agar Base Gal/Raf JP6313-270G 54
Minimal SD Base Gal/Raf JP6314-185G 37
Single Dropout Supplements (SDO)

单一省却成分添加剂

DO Supplement -His JP6316-10G 1.29
DO Supplement -Leu JP6317-10G 1.29
DO Supplement -Trp JP6318-10G 1.29
DO Supplement -Ura JP6319-10G 1.29
Double Dropout Supplements (DDO)

双省却成分添加剂

DO Supplement -His/-Leu JP6320-10G 1.19
DO Supplement -His/-Ura JP6321-10G 1.27
DO Supplement -Leu/-Trp JP6322-10G 1.19
DO Supplement -Met/-Trp JP6323-10G 1.27
DO Supplement -Trp/-Ura JP6324-10G 1.27
DO Supplement -Trp/-His JP6325-10G 1.27
DO Supplement -Ura/-Leu JP6326-10G 1.27
Triple Dropout Supplements (TDO)

三省却成分添加剂

DO Supplement -His/-Leu/-Trp JP6331-10G 1.17
DO Supplement -His/-Leu/-Ura JP6332-10G 1.17
DO Supplement -His/-Trp/-Ura JP6333-10G 1.25
DO Supplement -Leu/-Met/-Trp JP6334-10G 1.17
DO Supplement -Leu/-Trp/-Ura JP6335-10G 1.17
Quadruple Dropout Supplements (QDO)

四省却成分添加剂

DO Supplement -Ade/-His/-Leu/-Trp JP6341-10G 1.15
DO Supplement -His/-Leu/-Met/-Trp JP6342-10G 1.15
DO Supplement -His/-Leu/-Trp/-Ura JP6343-10G 1.15

 

 — —Written/Edited by V. Shallan【版权归金畔生物/JP金畔所有】

 

规格信息

品牌:

Jinpan

CAS:

N/A

规格:

10g

货期:

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