Sulforhodamine 101 磺酰罗丹明101

Sulforhodamine 101 磺酰罗丹明101

货号: JP4461-25MG 品牌: Jinpan 标签: 细胞生物学研究

描述

Sulforhodamine 101 磺酰罗丹明101

产品标签

Sulforhodamine 101 (SR101) 磺酰罗丹明101;Astrocyte marker 星形胶质细胞标志物;Octadecyl Rhodamine B(R18);2-photon microscopy 双光子荧光显微镜;CAS:82354-19-6;

产品信息

产品名称 产品编号 CAS NO. 规格 价格(元)
Sulforhodamine 101 磺酰罗丹明101 JP4461-25MG 60311-02-6 25mg 450

产品描述

磺酰罗丹明101(Sulforhodamine 101,CAS:60311-02-6)是一种水溶性,非固定的红色荧光极性示踪剂,主要用于神经元细胞形态,细胞-细胞通信和后续的噬菌体-溶酶体摄取和融合研究。与磺酰罗丹明B相比,具有更长的吸收波长。与磺酰罗丹明B和G相似,适用于肿瘤药物筛选。磺酰罗丹明101是一种非常理想的星形胶质细胞特异性标志物,以及一种活性依赖性的探针用来监测调节型胞吐作用。磺酰罗丹明101是罗丹明101磺酰氯,即德克萨斯红(Texas Red)的前体。

产品特性

1) CAS NO:60311-02-6

2) 化学名:9-(2,4-disulfophenyl)-2,3,6,7,12,13,16,17-octahydro-1H,5H,11H,15H-xantheno[2,3,4-ij:5,6,7-i’j’] diquinolizin-18-ium, inner salt

3) 同义名:SR 101;

4) 分子式:C31H30N2O7S2

5) 分子量:606.71 g/mol

6) 纯度:≥98%

7) 外观:深绿色至棕色至黑色粉末

8) 溶解性:溶于甲醇(1mg/ml),乙醇,DJPO(25mg/ml),水(20mg/ml)

9) Ex/Em:586/605 nm(in H2O)

10)化学结构式:Sulforhodamine 101 磺酰罗丹明101

保存与运输方法

保存:室温避光干燥保存,可置于-20℃长期保存,至少1年有效。

运输:室温运输。

使用实例【来自公开文献,仅做参考】

文献1:Rasmussen R et al. Sulforhodamine 101, a widely used astrocyte marker, can induce cortical seizure-like activity at concentrations commonly used. Sci Rep. 2016 Jul 26;6:30433.

使用方法:SR101 (Molecular Probes, S359) was dissolved in Ringer solution to concentrations of 25 μM, 50 μM, 100 μM or 250 μM. The SR101 was kept on the cortical surface for 1, 10, 20 or 30 min, depending on the concentration used.

重要结论:To label astrocytes with SR101, concentrations no higher than 50 μM should be used for in vivo experiments.

文献2:Schnell C et al. Active sulforhodamine 101 uptake into hippocampal astrocytes. PLoS One. 2012;7(11):e49398. PMID: 23189143

使用方法:Sulforhodamine 101 (SR101) labeling was performed with a low concentration of the dye [6], [19]. If not stated otherwise, slices were incubated for 20 minutes in carbogen-saturated aCSF containing 1 µM SR101 at 34°C. Thereafter, slices were incubated in aCSF for 10 minutes at 34°C to allow washout of excess dye from the extracellular space. Sodium-reduced solution was prepared by replacing NaCl by cholineCl.

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规格信息

品牌:

Jinpan

CAS:

60311-02-6

规格:

25mg

货期:

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