同仁化学Hampton蛋白结晶试剂盒LCP Sandwich Set| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Paul Marienfeld > LCP Sandwich Set

LCP Sandwich Set

Applications

  • Crystallization screening of membrane proteins in lipidic mesophase as well as bicelle method and batch method

Features

  • Small volume LCP, bicelle and microbatch crystallization
  • High quality glass optics
  • Excellent drop optics since mesophase bolus is physically sandwiched between two optically clear surfaces eliminating the mesophase / aqueous medium interface and the corresponding roughness
  • Glass plate allows for visualization of microcrystals using birefringence-free examination between crossed polarizers
  • Superhydrophobic glass surfaces
  • Optimized for bright field, UV and fluorescent microscopy
  • Excellent stability, resistant to evaporation

Description

The LCP Sandwich Set consists of a base glass slide and an optimized cover slip. LCP Sandwich Set developed jointly with the renowned Scripps Research Institute in La Jolla, California, USA and is manufactured by Paul Marienfeld (0890003).

The LCP Glass Sandwich Set is a specially designed plate for either automated or manual setting of 96 Lipidic Cubic Phase matrix screening experiments. The plate can also be used for the bicelle method and batch method. The thin (< 2 mm high) plates have exquisite optical properties and are well suited for the detection of microcrystals and for birefringence-free imaging between crossed polarizers. The plates allow for in meso crystallization trials with 50 nanoliters protein/lipid mesophase and 1 microliter precipitant solution per trial.

The 96 well LCP Glass Sandwich Plate consists of a) 127.8 x 85.5 x 1 mm glass base plate with the footprint of an SBS-compliant plate, a 140 µm thick double sticky spacer with 96 punched out holes and b) a 0.2 mm thick glass coverslip. The double sticky spacer is already adhered to the base plate. A brown paper liner covers and protects the top of the double sticky spacer and base plate. The 0.2 mm thick glass coverslip fits over and seals the entire 96 well lower plate. Alternatively a series of twenty-four siliconized 18 x 18 mm No. 1 square cover slides (HR3-152) can be used to seal the entire 96 well lower plate. Each 18 x 18 mm No. 1 cover slide seals 4 wells. Space for a bar code is available at the left end of the plate. Each well can contain 50 nanoliters of cubic phase and 1 microliter of crystallization reagent.

18 x 18 mm No. 1 Siliconized Cover Slides are made from chemically resistant borosilicate glass D 263 M of the first hydrolytic class. The slides are colorless, clear, and suitable for fluorescence microscopy. The slides feature a super hydrophobic surface on both sides. The slides are No. thickness (0.13 ro 0.16 mm). The slides are supplied in a two compartment plastic box, 100 slides per box, 10 boxes per case, 1,000 slides total for HR3-152.

The Tungsten-carbide glass cutter can be used for cutting and removal of the upper coverglass that seals the LCP Sandwich Set. Sold separately.

Show More



Hampton蛋白结晶试剂盒LCP Sandwich Set

Click to Zoom In

Hampton蛋白结晶试剂盒LCP Sandwich Set
Hampton蛋白结晶试剂盒LCP Sandwich Set
Hampton蛋白结晶试剂盒LCP Sandwich Set
Hampton蛋白结晶试剂盒LCP Sandwich Set
Hampton蛋白结晶试剂盒LCP Sandwich Set

CAT NO

HR3-151

NAME

LCP Sandwich Set

DESCRIPTION

Pack of 20

PRICE

$458.00

cart quote

CAT NO

HR3-152

NAME

18 x 18 mm No. 1 Siliconized Cover Slides, Squares

DESCRIPTION

Case of 10 packs

PRICE

$322.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒LCP Sandwich Set HR3-151 LCP Sandwich Set User GuideHampton蛋白结晶试剂盒LCP Sandwich Set HR3-151 LCP Sandwich Set Dimension Drawing

Related Item(S)

  • 4 Inch Soft Rubber Brayer
  • Tungsten Carbide Scribe & Glass Cutter with Replaceable Tips

References

1. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. Vadim Cherezov and Martin Caffrey. J. Appl. Cryst. (2003). 36, 1372-1377.

2. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Vadim Cherezov, Avinash Peddi, Lalitha Muthusubramaniam, Yuan F. Zheng and Martin Caffrey. Acta Cryst. (2004). D60, 1795-1807 DOI: 10.1107/S0907444904019109.

3. Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. Faham, S. & Bowie, J. U. (2002). J. Mol. Biol. 316, 1-6.

同仁化学Hampton蛋白结晶试剂盒FRAP Sandwich Set| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Paul Marienfeld > FRAP Sandwich Set

FRAP Sandwich Set

Applications

  • FRAP – rapidly screen diffusion properties of membrane proteins in LCP under variety of crystallization conditions

Features

  • Small volume FRAP
  • Superhydrophobic Glass Cover Slip and Glass Bottom Slide
  • Bottom Slide with 60 micron thick adhesive film and 96 sample positions
  • High quality glass optics
  • Excellent stability, resistant to evaporation
  • Optimized for bright field, UV and fluorescent microscopy

Description

FRAP (Fluorescence recovery after photobleaching) measures membrane protein mobility in lipids in a high throughput fashion. The system utilizes an automated high throughput LCP-FRAP assay to prescreen membrane protein crystallization conditions.

The FRAP assay can be used to pre-screen multiple protein constructs, lipids and additives to eliminate those conditions that are non-conducive to protein diffusion, and thus crystallization, from the subsequent crystallization trials. High protein mobility and fast diffusion rates correlate well with crystallization conditions.

The FRAP sandwich set consists of a base glass slide with sealing film and an optimized cover slip. This product has been developed jointly with the renowned Scripps Research Institute in La Jolla, California, USA.

The FRAP Sandwich Set is optimized for the pre-crystallization study of membrane proteins.

Developed at the NIH Roadmap Center for membrane protein research and routinely used in GPCR structural biology.

Bottom Slide Features
• Dimensions: approx 127.8 x 85.5 mm, thickness approx. 1 mm
• Superhydrophobic glass surface
• Covered with a 0.06 mm (60 micron) high spacer. This spacer has 96 recesses of 5 mm diameter. They are consistent with the SBS format for robotic handling. The spacer has an adhesive surface to which the hydrophobic coverslip attaches. The crystallization drops are, hence, sealed in these reaction chambers.

Cover Slip Features
• Dimensions: approx 112 x 77 mm, thickness No. 1.5 (0.16 to 0.19 mm)
• Superhydrophobic glass surface
• Optimized for bright field, UV and fluorescent microscopy

The FRAP Sandwich Set is compatible with the Formulatrix FRAP.

The FRAP Sandwich Set consists of 20 Superhydrophobic Cover Slips and 20 Superhyrophobic Bottom Slides with applied sealing film.

Show More



Hampton蛋白结晶试剂盒FRAP Sandwich Set

Click to Zoom In

Hampton蛋白结晶试剂盒FRAP Sandwich Set
Hampton蛋白结晶试剂盒FRAP Sandwich Set
Hampton蛋白结晶试剂盒FRAP Sandwich Set

CAT NO

HR3-144

NAME

FRAP Sandwich Set

DESCRIPTION

Pack of 20

PRICE

$458.00

cart quote

Related Item(S)

  • 4 Inch Soft Rubber Brayer
  • Tungsten Carbide Scribe & Glass Cutter with Replaceable Tips

References

1. F. Xu, W.Liu, M. A. Hanson, R. C. Stevens and V. Cherezov (2011) Development of an automated high throughput LCP-FRAP assay to guide membrane protein crystallization in lipid mesophases Cryst Growth Des 11: 1193-1201

2. Cherezov, V., Liu, J., Griffith, M., Hanson, M.A. and R. C. Stevens (2008) LCP-FRAP Assay for Pre-Screening Membrane Proteins for in Meso Crystallization. Cryst Growth Des 8: 4307-4315.