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Products > Crystallization Plates, Hardware & Accessories > Micro-Bridges > Micro-Bridges

Micro-Bridges

应用

  • Sitting drop crystallization
  • Heavy atom soaks
  • Seeding
  • 坐滴结晶
    重原子浸泡
    播种

特征

  • Perform sitting drop experiments in 24 well hanging drop plates
  • Removable
  • 在 24 孔悬挂滴板中进行坐滴实验
    可拆卸的

描述

Micro-Bridges are small devices in the shape of a bridge that are designed to carry out sitting drop vapor diffusion crystallization when placed in a VDX™ Plate or Linbro® Plate. A single Micro-Bridge fits neatly into the reservoir of a standard 24 well VDX Plate for a sitting drop crystallization experiment. Once placed inside the wells, Micro-Bridges are stable and there is no need to stick them to the wells with grease or adhesive. It is therefore possible to transfer them to other wells during or after a crystallization experiment.

Why sitting drop? Placing the droplet in the indentation greatly reduces the risk of losing the protein by accident. Crystallization can be carried out in the presence of detergents and organic solvents which are compatible with polystyrene (such as MPD, iso-propanol, and ethanol). The protein drop is less affected by condensation problems. Soaking and seeding experiments can be carried out easily. Crystals can be transported more securely. Larger drop volumes can be used. Micro-Bridges have a concave indentation in the top surface of the bridge which holds the sample droplet during a crystallization experiment and prevents the droplet from spreading over a large area. Made from polystyrene, these parts are highly transparent and suitable for most crystallizations. The surface of the indentation is highly polished to facilitate the visual inspection of the drops under a microscope. The maximum drop volume for the Micro-Bridge is 35 µl. Reservoirs can be sealed with plain cover slides and vacuum grease or clear sealing tape.

Approximate dimensions: Height: 12 mm Length: 14 mm Width: 7 mm

微桥是桥形的小型设备,设计用于在放置在 VDX™ 板或 Linbro® 板中时进行坐滴蒸汽扩散结晶。单个 Micro-Bridge 可巧妙地装入标准 24 孔 VDX 板的储液器中,用于坐滴结晶实验。一旦放置在孔内,Micro-Bridges 就很稳定,无需用油脂或粘合剂将它们粘在孔上。因此,可以在结晶实验期间或之后将它们转移到其他孔中。

为什么要坐下来?将液滴放入压痕中可大大降低意外丢失蛋白质的风险。可以在与聚苯乙烯相容的去污剂和有机溶剂(如 MPD、异丙醇和乙醇)存在下进行结晶。蛋白质下降受冷凝问题的影响较小。可以轻松进行浸泡和播种实验。水晶可以更安全地运输。可以使用更大的液滴体积。微桥在桥的顶部表面有一个凹形压痕,用于在结晶实验期间固定样品液滴并防止液滴在大面积上扩散。这些部件由聚苯乙烯制成,高度透明,适用于大多数结晶。压痕表面经过高度抛光,便于在显微镜下目视检查液滴。 Micro-Bridge 的最大滴体积为 35 µl。可以使用普通盖玻片和真空润滑脂或透明密封胶带密封储液罐。

大概尺寸: 高度:12 毫米 长度:14 毫米 宽度:7 毫米

HR3-310/HR3-312 Micro-Bridges

Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312
Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312
Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312
Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312
Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312
Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312

CAT NO

HR3-310

NAME

Micro-Bridges

DESCRIPTION

100 pack

PRICE

$86.00

CAT NO

HR3-312

NAME

Micro-Bridges

DESCRIPTION

400 pack

PRICE

$297.00

支持材料

Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312 HR3-310 HR3-312 Micro-Bridges User Guide

参考

1. Structure of aluminum-bound ovotransferrin at 2.15 angstrm resolution. K, Mizutani, B. Mikami, S. Aibara, and M. Hirose. Acta Cryst (2005) D61, 1636-1642.

1. 2.15 埃分辨率下铝结合卵转铁蛋白的结构。 K、Mizutani、B. Mikami、S. Aibara 和 M. Hirose。 水晶学报 (2005) D61, 1636-1642。

同仁化学Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342| 日本DOJINDO

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Crystallization Plates, Hardware & Accessories > Micro-Bridges > Micro-Bridges Polypropylene

Micro-Bridges Polypropylene

应用

  • Sitting drop crystallization
  • Heavy atom soaks
  • Small molecule crystallization
  • 坐滴结晶
    重原子浸泡
    小分子结晶

特征

  • Perform sitting drop experiments in 24 well hanging drop plates
  • Removable
  • 在 24 孔悬挂滴板中进行坐滴实验
    可拆卸的

描述

Same great Micro-Bridges, but manufactured from clarified polypropylene. These Micro-Bridges are resistant to most organic solvents, and are especially useful with crystallization experiments that involve detergents and other hydrophobic reagents. They resist drop-spreading typically observed when using reagents such as detergents, volatile organics such as iso-propanol and ethanol, and non-volatile organics such as MPD. These Micro-Bridges also resist acetone, dioxane, acetonitrile, 2,2,2-trifluoroethanol, and other aggressive organic solvents.

Approximate dimensions: Height: 12 mm Length: 14 mm Width: 7 mm

同样出色的微型桥接器,但由澄清的聚丙烯制成。 这些微桥对大多数有机溶剂具有耐受性,尤其适用于涉及去污剂和其他疏水性试剂的结晶实验。 当使用诸如去污剂、挥发性有机物(例如异丙醇和乙醇)以及非挥发性有机物(例如 MPD)等试剂时,它们通常会观察到液滴扩散。 这些微桥还可以抵抗丙酮、二恶烷、乙腈、2,2,2-三氟乙醇和其他腐蚀性有机溶剂。

大概尺寸: 高度:12 毫米 长度:14 毫米 宽度:7 毫米

Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342
Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342
Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342
Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342
Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342
Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342

CAT NO

HR3-340

NAME

Micro-Bridges Polypropylene

DESCRIPTION

100 pack

PRICE

$98.00

CAT NO

HR3-342

NAME

Micro-Bridges Polypropylene

DESCRIPTION

400 pack

PRICE

$334.00

支持材料

Hampton蛋白结晶试剂盒Micro-Bridges Polypropylene/HR3-340/HR3-342 Micro-bridges User Guide