Fructosyl-peptide Oxidase (FPOX-CE) 果糖基 – 缩氨酸氧化酶| 龟甲万百欧凯米发株式会社

上海金畔生物科技有限公司代理龟甲万酶制剂全线产品,欢迎访问官网了解更多信息。

Fructosyl-peptide Oxidase (FPOX-CE) 果糖基 – 缩氨酸氧化酶

  • 临床检测用酶

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

由来 recombinant E. coli
系统名称

Fructosyl-peptide : oxygen oxidoreductase

EC编号 1.5.3
反应式

Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2

规格

Appearance yellow lyophilizate
Activity

≧6.0 U/mg

Contaminants Catalase ≦1.0 U/U%
Stabilizer ethylenediaminetetraacetic acid (EDTA), sodium glutamate
Storage condition below -20℃ protected from light

特性

Molecular weight ca. 60kDa (gel filtration)
Structure monomer of 52 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 3.4×10-3M (fructosyl-valyl-histidine)
4.4×10-3M (fructosyl-glycine)
8.9×10-3M (Nε-fructosyl-lysine)
pH Optimum 7.5–8.0
pH Stability 6.0–9.5
Optimum temperature 35–42℃
Thermal stability below 45℃
Stability (powder form) stable at 37℃ for at least one month
Specificity fructosyl-valyl-histidine (100), fructosyl-glycine (53)
Nε-fructosyl-lysine (84)

适用类型

The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.

参考文献

Hirokawa K, Gomi K, Bakke M, Kajiyama N. (2003)
Distribution and properties of novel deglycating enzymes for fructosyl peptide in fungi.
Archives of Microbiology, 180, 227-231.

Hirokawa K, Gomi K, Kajiyama N. (2003)
Molecular cloning and expression of novel fructosyl peptide oxidases and their application for the measurement of glycated protein.
Biochem Biophys Res Commun, 311(1), 104-111.

Hirokawa K, Shimoji K, Kajiyama N. (2005)
An enzymatic method for the determination of hemoglobinA1C.
Biotechnology Letters, 27(14), 963-968.

  • 产品单页
  • SDS