GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒 GFP-CERTIFIED® Apoptosis/Necrosis detection kit

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GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒                              GFP-CERTIFIED® Apoptosis/Necrosis detection kit

GFP-CERTIFIED® Apoptosis/Necrosis detection kit

可进行区分正常细胞、早期凋亡细胞、晚期凋亡细胞和坏死细胞的多重检测,可与 GFP 和其他绿色荧光探针兼容。

 

● 可以兼容 GFP 和其他绿色荧光探针

● 容易区分正常细胞、早期凋亡细胞、晚期凋亡细胞和坏死细胞

● 已根据荧光显微镜和流式细胞仪等应用进行过优化

● 适用于死亡途径分析及药物、毒素研究

 

从凋亡到坏死的过渡是一个松散定义的连续体。质膜细胞外表面上磷脂酰丝氨酸(PS)的暴露仍是早期细胞凋亡的共同标志。这时候在Ca2+存在下,磷脂结合蛋白例如 Annexin V 与 PS 具有亲和力。由于 Annexin V 不具有细胞渗透性,所以这种胞外PS的结合是早期凋亡细胞对具有选择性的。

同样,质膜完整性的丧失,如不具有膜渗透性DNA嵌入染料若能标记出细胞核,则是表明晚期细胞凋亡和坏死的直接方法。考虑到Annexin V通常与荧光素(又称FITC)偶联,检测绿色荧光蛋白(GFP)表达细胞中的凋亡时可能会出现问题。以上两个荧光团的发射图谱会发生光谱重叠,使信号之间的区分变得困难或不可能,从而损害了总体上的数据质量。GFP-CERTIFIED® 细胞凋亡/坏死检测系统专为表达GFP的细胞系以及表达蓝色或青色荧光蛋白(BFP、CFPs)的细胞而设计,同时也可以应用于不带荧光蛋白的细胞的凋亡和坏死的细胞。另外,本试剂盒适合用于活细胞或结合了探针(如标记抗体、或与荧光素或香豆素显示相似光谱特性的其他荧光偶联物)的细胞。本试剂盒包含了用于确定细胞凋亡及坏死的早期和晚期阶段中所需的全部试剂。Annexin V -EnzoGold(增强型Cyanine-3)偶联物能够检测不同于荧光素或GFP的细胞凋亡。坏死检测试剂(红色)类似于发出红色的染料7-AAD,可促进晚期细胞凋亡和坏死检测。本试剂盒还包含了可用作阳性对照的凋亡诱导剂(Staurosporine)。检测试剂可单独使用或分别与其他单独或多重应用组合使用。本试剂盒还可以通过流式细胞仪和荧光/共焦显微镜提供快速、准确的检查结果。

GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒                              GFP-CERTIFIED® Apoptosis/Necrosis detection kit

GFP、Annexin V-Cyanine 3 偶联物和坏死检测试剂(红色)的激发(阴影线)和发光(固体)光谱。

三种染料都可以轻易地被 488 nm 激光源激发。所有荧光团之间的发射峰值容易区分。

GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒                              GFP-CERTIFIED® Apoptosis/Necrosis detection kit

GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒(ENZ-51002



GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒                              GFP-CERTIFIED® Apoptosis/Necrosis detection kit


流式细胞仪。用0.2%DMSO(panel A)模拟诱导 Jurkat 细胞,或用 2 μM 星形孢菌素(panel B)在37°C下诱导 Jurkat 细胞4h。经过处理后,在含有 Annexin V-Cyanine 3 和坏死检测试剂(红色;一种远红色发光的 DNA 嵌入染料)的缓冲液中孵育细胞,然后用 488 nm 激光的流式细胞仪进行分析,用 FL2(细胞凋亡)和 FL3(坏死)通道进行荧光检测。

模拟诱导的细胞凋亡和坏死主要呈阴性。处理4h 后,会出现3种细胞群:

(1)可以存活且不凋亡、不坏死的细胞(Annexin V-Cyanine 3、坏死检测试剂呈阴性);

(2)正在经历凋亡的细胞(Annexin V-Cyanine 3 呈阳性和 NDR 呈阴性);

(3)处于晚期凋亡和早期坏死的细胞(Annexin V-Cyanine 3 和坏死检测试剂呈阳性)。

 


GFP-CERTIFIED® 细胞凋亡/坏死检测试剂盒                              GFP-CERTIFIED® Apoptosis/Necrosis detection kit

用 2.5 µM 星形孢菌素刺激 Jurkat 细胞 0-6 h。使用 Jurkat 悬浮细胞的流式细胞仪结果表现为早期凋亡(Annexin V-EnzoGold)和晚期凋亡/坏死(Annexin V-EnzoGold 和坏死染色)。



◆产品规格


 应用:

 流式细胞仪、荧光显微镜、荧光检测。

 使用事项/稳定性:

 Annexin V-EnzoGold 和结合缓冲液应在4°C储存。
 其他全部试剂应在-20°C储存。
 重组诱导剂(星形孢菌素)应在-20°C下储存。具体请参阅说明书。

 储存条件:

 避光。

 运输:

 蓝冰运输。

 短期储存温度:

 4°C

 试剂盒构成:

 凋亡检测试剂 (Annexin   V-EnzoGold)
 坏死检测试剂
 凋亡诱导剂(Staurosporine)
 结合缓冲液(10X)

 技术信息/产品说明:

 应用说明:
 Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential 

 Neurotoxicants
 引用样本:
 For an overview on cited samples please click here.

 监管状况:

 RUO-仅供研究用



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参考文献


 1.

Inhibitory role of TRIP-Br1/XIAP in necroptosis under nutrient/serum starvation: Z. Sandag, et al.; Mol. Cells 43, 236 (2020), 摘要全文

 2.

Inhibitory role of AMP‑activated protein kinase in necroptosis of HCT116 colon cancer cells with p53 null mutation under nutrient starvation: D.T. Le, et al.; Int. J. Oncol. 54, 702 (2019), 摘要;


 3.

Injectable and Quadruple-Functional Hydrogel as an Alternative to Intravenous Delivery for Enhanced Tumor Targeting: Z.Q. Zhang, et al.; ACS Appl. Mater. Interfaces 11, 34634 (2019), 摘要;


 4.

Intrinsic antibacterial activity of nanoparticles made of β-cyclodextrins potentiates their effect as drug nanocarriers against tuberculosis: A. Machelart, et al.; ACS Nano 13, 3992 (2019), 摘要;


 5.

Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells: Y. Zhu, et al.; Oncogene 38, 5778 (2019), Application(s): Flow cytometry using MCF-7 cells, 摘要;


  6.

PKM2 coordinates glycolysis with mitochondrial fusion and oxidative phosphorylation: T. Li, et al.; Protein Cell 10, 583 (2019), Application(s): Flow cytometry using H1299 and HepG2 cells, 摘要;

 

 7.

Tamoxifen acts on Trypanosoma cruzi sphingolipid pathway triggering an apoptotic death process: M. Landoni, et al.; Biochem. Biophys. Res. Commun. 516, 934 (2019), 摘要;

 

 8.

Anti-leukemic efficacy of BET inhibitor in a preclinical mouse model of MLL-AF4+ infant ALL: M. Bardini, et al.; Mol. Cancer Ther. 17, 1705 (2018), 摘要;

 

 9.

Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5: C. Angelucci, et al.; Oncotarget. 9, 24364 (2018), Application(s): Fluorescent microscopy with MCF-7 cells, 摘要全文

 

10.

Preclinical efficacy and safety of CD19CAR cytokine-induced killer cells transfected with Sleeping Beauty transposon for the treatment of acute lymphoblastic leukemia: C.F. Magnani, et al.; Hum. Gene. Ther. 29, 605 (2018), 摘要;


11.

Protective effect of a newly developed fucose-deficient recombinant antithrombin against histone-induced endothelial damage: T. Iba, et al.; Int. J. Hematol. 107, 528 (2018), 摘要;


12.

The poly (ADP-ribose) polymerase inhibitor olaparib induces up-regulation of death receptors in primary acute myeloid leukemia blasts by NF-κB activation: I. Faraoni, et al.; Cancer Lett. 423, 127 (2018), Application(s): Flow cytometry with primary AML cells, 摘要;


13.

Toxicity and phototoxicity in human ARPE-19 retinal pigment epithelium cells of dyes commonly used in retinal surgery: D. Awad, et al.; Eur. J. Ophthalmol. 28, 433 (2018), 摘要全文


14.

Cluster microRNAs miR‐194 and miR‐215 suppress the tumorigenicity of intestinal tumor organoids: T. Nakaoka, et al.; Cancer Sci. 108, 678 (2017), Application(s): Flow cytometry using organoid culture of mouse intestinal tumors, 摘要

15.

Elaidic Acid, a Trans-Fatty Acid, Enhances the Metastasis of Colorectal Cancer Cells: H. Ohmori, et al.; Pathobiology 84, 144 (2017), 摘要;


16.

Metabolic modulation of Ewing sarcoma cells inhibits tumor growth and stem cell properties: A. Dasgupta, et al.; Oncotarget. 8, 77292 (2017), Application(s): Flow cytometry with A673 cells, 摘要全文


17.

Multiple hyperthermia-mediated release of TRAIL/SPION nanocomplex from thermosensitive polymeric hydrogels for combination cancer therapy: Z.Q. Zhang, et al.; Biomaterials. 132, 16 (2017), Application(s): Confocal microscopy with PC3 and U-87 MG cells, 摘要;


18.

Pro-metastatic intracellular signaling of the elaidic trans fatty acid: K. Fujii, et al.; Int. J. Oncol. 50, 85 (2017), 摘要;


19.

Visualization of ceramide channels in lysosomes following endogenous palmitoyl-ceramide accumulation as an initial step in the induction of necrosis: M. Yamane, et al.; Biochem. Biophys. Rep. 11, 174 (2017), Application(s): Confocal microscopy with A549 cells, 摘要全文


20.

Immunotherapy of acute leukemia by chimeric antigen receptor-modified lymphocytes using an improved Sleeping Beauty transposon platform: C.F. Magnani, et al.; Oncotarget 7, 51581 (2016), Application(s): Flow cytometry using KG-1, REH, Nalm-6, and THP-1 cells, 摘要全文


21.

Novel long chain fatty acid derivatives of quercetin-3-O-glucoside reduce cytotoxicity induced by cigarette smoke toxicants in human fetal lung fibroblasts: N. Sumudu, et al.; Eur. J. Pharmacol. 781, 128 (2016), 摘要;


22.

Phlorofucofuroeckol Improves Glutamate-Induced Neurotoxicity through Modulation of Oxidative Stress-Mediated Mitochondrial Dysfunction in PC12 Cells: J.J. Kim, et al.; PLoS One 11, e0163433 (2016), 摘要全文


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PKLR promotes colorectal cancer liver colonization through induction of glutathione synthesis: A. Nguyen, et al.; J. Clin. Invest. 126, 681 (2016), Application(s): Assessed Apoptosis, Flow cytometry, 摘要

24.

Protective effects of β‐casofensin, a bioactive peptide from bovine β‐casein, against indomethacin‐induced intestinal lesions in rats: C. Bessette, et al.; Mol. Nutr. Food Res. 60, 823 (2016), 摘要;


25.

BRCA1, PARP1 and γH2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib: I. Faraoni, et al.; Biochim. Biophys. Acta 1852, 462 (2015), Application(s): Flow cytometry using primary AML cells, 摘要;


26.

Growth-promoting and tumourigenic activity of c-Myc is suppressed by Hhex: V. Marfil, et al.; Oncogene 34, 3011 (2015), Application(s): Flow cytometry using HO15.19 and TGR-1 cells, 摘要;


27.

common set of proteins modulated in Chikungunya virus infection: R. Abraham, et al.; J. Proteomics. 120, 126 (2015), Application(s): Flow cytometry using HEK293 cells, 摘要;


28.

Identification of novel osteogenic compounds by an ex-vivo sp7: luciferase zebrafish scale assay: E. de Vrieze, et al.; Blood 74, 106 (2015), Application(s): Fluorescence microscopy using fish scales, 摘要;


29.

Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells: M.J. Koo, et al.; Biochem. Biophys. Res. Commun. 464, 875 (2015), Application(s): Apoptosis/necrosis assay, 摘要;


30.

Influenza virus M2 targets cystic fibrosis transmembrane conductance regulator for lysosomal degradation during viral infection: J.D. Londino, et al.; FASEB J. 29, 2712 (2015), Application(s): Flow cytometry using HEK293 cells, 摘要

31.

Inhibition of autophagy exerts anti-colon cancer effects via apoptosis induced by p53 activation and ER stress: K. Sakitani, et al.; BMC Cancer 15, 795 (2015), Application(s): Flow cytometric analysis of apoptosis, 摘要全文


32.

N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting: P.H. Lin, et al.; J. Biomed. Sci. 22, 44 (2015), Application(s): Fluorescence microscopy using HaCaT and HEK293 cells, 摘要全文

 

33.

Novel carbocyclic curcumin analog CUR3d modulates genes involved in multiple apoptosis pathways in human hepatocellular carcinoma cells.: K.S. Bhullar, et al. ; Chem. Biol. Interact. 242, 107 (2015), Application(s): Fluorescence microscopy, 摘要;


34.

NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival: J.M. Calandria, et al.; Cell Death Differ. 22, 1363 (2015), Application(s): Assay, 摘要;


35.

ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox: D. Zhang, et al.; Plant Cell Rep. 34, 1499 (2015), 摘要;

 

36.

The Adhesion GPCR CD97/ADGRE5 inhibits apoptosis: C.C. Hsiao, et al.; Int. J. Biochem. Cell. Biol. 65, 197 (2015), Application(s): Flow cytometry using HT1080 cells, 摘要;


37.

The effect of plasma-derived activated protein C on leukocyte cell-death and vascular endothelial damage: T. Iba, et al.; Thromb. Res 135, 963 (2015), Application(s): Fluorescence Microscopy using leukocytes, 摘要;


38.

Accumulation of cytosolic calcium induces necroptotic cell death in human neuroblastoma: M. Nomura, et al.; Cancer Res. 74, 1056 (2014), 摘要;


39.

Apoptotic and inhibitory effects on cell proliferation of hepatocellular carcinoma HepG2 cells by methanol leaf extract of Costus speciosus: S.V. Nair, et al.; Biomed. Res. Int. 2014, Article ID 637098 (2014), Application(s): Measurement by flow cytometry, 摘要全文


40.

Combination of antithrombin and recombinant thrombomodulin modulates neutrophil cell-death and decreases circulating DAMPs levels in endotoxemic rats: T. Iba, et al.; Throm. Res. 134, 169 (2014), Application(s): Detection Kit using live cells, 摘要;


41.

Fatty acid esters of ohloridzin induce apoptosis of human liver cancer cells through altered gene expression: S.V. Nair, et al.; PLoS One 9, e107149 (2014), Application(s): Fluorescence microscopy of HepG2 cells, 摘要全文


42.

Flavonoid-enriched apple fraction AF4 induces cell cycle arrest, DNA topoisomerase II inhibition, and apoptosis in human liver cancer HepG2 cells: S. Sudan, et al.; Nutr. Cancer 66, 1237 (2014), 摘要;


43.

Quercetin-3-O-glucoside induces human DNA topoisomerase II inhibition, cell cycle arrest and apoptosis in hepatocellular carcinoma cells: S. Sudan, et al.; Anticancer Res. 34, 1691 (2014), 摘要;


44.

A light-activated NO donor attenuates anchorage independent growth of cancer cells: Important role of a cross talk between NO and other reactive oxygen species: S. Sen, et al.; Arch. Biochem. Biophys. 540, 33 (2013), Application(s): Detection Kit using live cells, 摘要;


45.

Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks: L.M. Holdt, et al.; PLoS Genet. 9, e1003588 (2013), Application(s): Detection Kit using live cells, 摘要

46.

Analysis of protein translocation into the endoplasmic reticulum of human cells: J. Dudek, et al.; Methods Mol. Biol. 1033, 285 (2013), 摘要;


47.

Characterizing virulence-specific perturbations in the mitochondrial function of macrophages infected with Mycobacterium tuberculosis: S. Jamwal, et al.; Sci. Rep. 3, 1328 (2013), 摘要

48.

Evaluation of anticancer effects and enhanced doxorubicin cytotoxicity of xanthine derivatives using canine hemangiosarcoma cell lines: T. Motegi, et al.; Res. Vet. Sci. 95, 600 (2013), Application(s): Fluorescence microscopy using canine HAS cells, 摘要;


49.

Genista sessilifolia DC. extracts induce apoptosis across a range of cancer cell lines: P. Bontempo, et al.; Cell Prolif. 46, 183 (2013), Application(s): Detection Kit using live cells, 摘要;


50.

High mobility group box 1 released from necrotic cells enhances regrowth and metastasis of cancer cells that have survived chemotherapy: Y. Luo, et al.; Eur. J. Cancer. 49, 741 (2013), Application(s): Fluorescence microscopy using CT26 cells, 摘要;


51.

Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells: M. Suenaga, et al.; Anticancer Res. 33, 1547 (2013), 摘要;


52.

Comparison of different suicide-gene strategies for the safety improvement of genetically manipulated T cells: V. Marin, et al.; Hum. Gene Ther. Methods 23, 376 (2012), Application(s): Flow cytometry using EBV-CTL and 293 T cells, 摘要全文


53.

Maintenance of higher H2O2 levels, and its mechanism of action to induce growth in breast cancer cells: Important roles of bioactive catalase and PP2A: S. Sen, et al.; Free Radic. Biol. Med. 53, 1541 (2012), Application(s): Detection Kit using live cells, 摘要;


54.

Mesenchymal stem cells from Shwachman–Diamond syndrome patients display normal functions and do not contribute to hematological defects: V. André, et al.; Blood Cancer J. 2, e94 (2012), Application(s): Flow cytometry using human neutrophils, 摘要

55.

Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine γ-lyase, an animal model of cystathioninuria: H. Yamada, et al.; Free Radic. Biol. Med. 52, 1716 (2012), Application(s): Fluorescence microscopy using mouse hepatocytes, 摘要;


56.

Molecular mechanism of interleukin-2-induced mucosal homeostasis : J. Mishra, et al.; Am. J. Physiol. Cell Physiol. 302, C735 (2012), Application(s): Detection Kit using live cells, 摘要全文


57.

Serratia marcescens induces apoptotic cell death in host immune cells via a lipopolysaccharide- and flagella-dependent mechanism: K. Ishii, et al.; J. Biol. Chem. 287, 36582 (2012), Application(s): Apoptosis detected in Silkworm larvae, 摘要;


58.

Serratia marcescens Induces Apoptotic Cell Death in Host Immune Cells via a Lipopolysaccharide- and Flagella-dependent Mechanism: K. Ishii, et al.; J. Biol. Chem. 287, 36582 (2012), Application(s): Detection Kit using live cells, 摘要全文


59.

Two mutations impair the stability and function of ubiquitin-activating enzyme (E1): T. Lao, et al.; J. Cell. Physiol. 227, 1561 (2012), Application(s): Viability and induction of cell death observed by confocal microscopy, 摘要全文


60.

Critical roles of Cold Shock Domain Protein A as an endogenous angiogenesis inhibitor in skeletal muscle: Y. Saito, et al.; Antioxid. Redox. Signal. 15, 2109 (2011), 摘要;


61.

Cell Surface Externalization of Annexin A1 as a Failsafe Mechanism Preventing Inflammatory Responses during Secondary Necrosis: K.E. Blume, et al.; J. Immunol. 183, 8138 (2009), 摘要全文


62. 

Supplemental Information for: Cell Surface Externalization of Annexin A1 as a Failsafe Mechanism Preventing Inflammatory Responses during Secondary Necrosis: K.E. Blume, et al.; J. Immunol. 183, (2009), (Supplemental Information), 全文

产品编号 产品名称 产品规格 产品等级
ENZ-51002-25 GFP-CERTIFIED® Apoptosis/Necrosis detection kit
细胞凋亡/坏死检测试剂盒(荧光显微镜&流式)(GFP细胞系)
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ENZ-51002-100 GFP-CERTIFIED® Apoptosis/Necrosis detection kit
细胞凋亡/坏死检测试剂盒(荧光显微镜&流式)(GFP细胞系)
100 assays